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KMID : 0624620110440020113
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BMB Reports
2011 Volume.44 No. 2 p.113 ~ p.117
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Identification of EphA7 BAC clone containing a long-range dorsal midline-specific enhancer
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Kim Yu-Jin
Park Soo-Chul
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Abstract
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Previous studies suggest that EphA7 plays a critical role in neural tube closure or cortical progenitor apoptosis. In this report, enhancer trap assay was used to modify various EphA7 BAC clones and screen a large genomic region spanning 570 kb downstream of the EphA7 gene. We found that the dorsal midline-specific EphA7 enhancer resides on the 457D20 EphA7 BAC clone and is localized to a 35 kb genomic region in between +345.7 kb to +379.8 kb downstream of the EphA7 transcription start site. Identification of the EphA7 BAC clone containing a long-range dorsal midline enhancer may constitute a useful tool for investigating the biological functions of EphA7 in vivo.
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KEYWORD
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Bacterial artificial chromosome, Enhancer, EphA7, Transgenic mice
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